解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Human papillomavirus (HPV) E6 oncoproteins target numerous cellular proteins for ubiquitin-mediated degradation. In the case of p53 this is mediated by the E6AP ubiquitin ligase. However, there are conflicting reports concerning how central E6AP is to the global function of the HPV-16 and HPV-18 E6 oncoproteins. To in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.029
更新日期:2009-10-10 00:00:00
abstract::The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.007
更新日期:2009-10-10 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::Interleukin (IL)-15, is a cytokine that is important for the maintenance of long-lasting, high-avidity T cell response to invading pathogens and has, therefore, been used in vaccine and therapeutic platforms as an adjuvant. In addition to pure protein delivery, plasmids encoding the IL-15 gene have been utilized. Howe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.017
更新日期:2009-10-10 00:00:00
abstract::The phosphorylated protein pp65 of human Cytomegalovirus (HCMV) is the predominant virion protein and the major tegument constituent. It plays important roles in HCMV infection and virion assembly. Live cell imaging and fluorescence recovery after photobleaching (FRAP) analysis showed that HCMV pp65 accumulated dynami...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.021
更新日期:2009-09-30 00:00:00
abstract::Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.010
更新日期:2009-09-30 00:00:00
abstract::Studies were performed to induce cross-clade neutralizing antibodies (Abs) by testing various combinations of prime and boost constructs that focus the immune response on structurally-conserved epitopes in the V3 loop of HIV-1 gp120. Rabbits were immunized with gp120 DNA containing a V3 loop characterized by the GPGR ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.039
更新日期:2009-09-15 00:00:00
abstract::Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immuno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.035
更新日期:2009-09-15 00:00:00
abstract::Hemorrhagic fever viruses are associated with rapidly progressing severe disease with high case fatality, making them of public health and biothreat importance. Effective antivirals are not available for most of the members of this diverse group of viruses. A broad spectrum strategy for antiviral development would be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.032
更新日期:2009-09-15 00:00:00
abstract::Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.007
更新日期:2009-08-15 00:00:00
abstract::Virus infection may contribute to asthma pathogenesis. In turn, a Th2-polarized pulmonary environment may increase host susceptibility to infection. We used a cockroach antigen (CRA) model of allergic airway disease to test the hypothesis that Th2 cytokine overproduction increases susceptibility to mouse adenovirus ty...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.009
更新日期:2009-08-15 00:00:00
abstract::H5 and H7 avian influenza viruses can become highly pathogenic in chickens after interspecies transmission. These viruses have transmitted directly to humans from birds in Eurasia and Africa (H5N1), the Netherlands (H7N7), and Canada (H7N3). Here we report antigenic, sequence, and phylogenetic analyses of H7N3 viruses...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.008
更新日期:2009-08-01 00:00:00
abstract::The virulence factor of Melon necrotic spot virus (MNSV), a virus that induces systemic necrotic spot disease on melon plants, was investigated. When the replication protein p29 was expressed in N. benthamiana using a Cucumber mosaic virus vector, necrotic spots appeared on the leaf tissue. Transmission electron micro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.012
更新日期:2009-08-01 00:00:00
abstract::Geographical separation of host species has shaped the avian influenza A virus gene pool into independently evolving Eurasian and American lineages, although phylogenetic evidence for gene flow and reassortment indicates that these lineages also mix on occasion. While the evolutionary dynamics of the avian influenza g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.002
更新日期:2009-08-01 00:00:00
abstract::The biological significance of protective CD8 T-cell-mediated responses against non-traditional alternative reading frame epitopes remains relatively unknown. Cytolytic CD8 T cells (CTL) specific for a non-traditional cryptic MHC class I epitope, SYNTGRFPPL, are critically involved in the protection of mice during inf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.003
更新日期:2009-08-01 00:00:00
abstract::The conserved nature of the epitopes of the four broadly neutralizing antibodies (BNAbs), b12, 2G12, 2F5, and 4E10, may imply that the sensitivity of HIV-1 for these BNAbs remains fairly constant over the course of infection. Here, we demonstrate that viruses isolated early during the course of infection were mostly s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.028
更新日期:2009-08-01 00:00:00
abstract::An ideal system to investigate individual determinants of the replication process of (+)-strand RNA viruses is a cell-free extract that supports viral protein and RNA synthesis in a synchronized manner. Here, we applied a translation/replication system based on cytoplasmic extracts of Nicotiana tabacum cells to Tomato...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.009
更新日期:2009-08-01 00:00:00
abstract::Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.020
更新日期:2009-07-20 00:00:00
abstract::When effector CD4+ T cells carrying integrated HIV-1 proviruses revert back to a resting memory state, the virus can remain silent in those cells for years. Following re-exposure to the nominal antigen or in response to other stimuli (e.g. pro-inflammatory cytokines), these cells can begin to produce virus. Here we de...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.019
更新日期:2009-06-20 00:00:00
abstract::Little is known about antigen-specific T-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. In the present study we have successfully induced an in vitro generation of virus-specific cytotoxic T-cells (CTLs) from isogeneic ginbuna crucian carp. Respond...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.008
更新日期:2009-06-20 00:00:00
abstract::The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant wer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.04.007
更新日期:2009-06-20 00:00:00
abstract::Cytotoxic T-lymphocyte (CTL) epitopes within the HIV genome are subject to negative and positive selective pressures, the balance of which influences CTL escape at a given epitope. We investigated whether viral fitness requirements dictate conservation of the HLA-A2 restricted immunodominant epitope SLYNTVATL (SL9). V...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.003
更新日期:2009-05-25 00:00:00
abstract::The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.007
更新日期:2009-05-25 00:00:00
abstract::Although a live attenuated HIV vaccine is not currently considered for safety reasons, a strategy inducing both T cells and neutralizing antibodies to native assembled HIV-1 particles expressed by a replicating virus might mimic the advantageous characteristics of live attenuated vaccine. To this aim, we generated a l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.047
更新日期:2009-05-25 00:00:00
abstract::Recently, cases of severe respiratory illness in military and civilian populations have been associated with a new genomic variant of adenovirus (Ad) serotype 14, designated Ad14a. Compared to the Ad14 reference strain (de Wit), this new virus had a deletion of two amino acid residues in the fiber protein knob. Here w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.034
更新日期:2009-05-10 00:00:00
abstract::HIV-1 variants resistant to small molecule CCR5 inhibitors such as vicriviroc (VVC) have modified Env complexes that can use both the inhibitor-bound and -free forms of the CCR5 co-receptor to enter target cells. However, entry via the inhibitor-CCR5 complex is inefficient in some, but not all, cell types, particularl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.044
更新日期:2009-05-10 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV-68) replicates robustly in cell culture, providing a model for studying viral genome replication during de novo infection of tumor-associated herpesviruses. We have previously identified a 1.25-kb origin of lytic replication (oriLyt) for MHV-68. To further investigate the molecular mech...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.029
更新日期:2009-05-10 00:00:00
abstract::Hepatitis B virus (HBV) clone 4B replicated much more efficiently than clone 2A of the same genotype. Introduction of its T1753C, A1762T, G1764A, and C1766T core promoter mutations into the 2A genome greatly enhanced genome replication and suppressed HBeAg expression. Here we show that these effects are mediated by tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.002
更新日期:2009-05-10 00:00:00
abstract::The HIV-1 transframe protein p6 known to modulate HIV-1 protease activation has been suggested to interact with the viral pathogenicity factor Nef. However, a potential interaction site in p6 has not been mapped so far. To evaluate effects of p6 modification on viral replication in light of Nef function, clustered sub...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.042
更新日期:2009-04-25 00:00:00
abstract::Hepatitis C virus (HCV) RNA replication requires viral nonstructural proteins as well as cellular factors. Recently, a cellular protein, synaptotagmin-binding, cytoplasmic RNA-interacting protein (SYNCRIP), also known as NSAP1, was found to bind HCV RNA and enhance HCV IRES-dependent translation. We investigate whethe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.018
更新日期:2009-04-10 00:00:00
abstract::The Rta (R transactivator) protein plays an essential role in the Epstein-Barr viral (EBV) lytic cascade. Rta activates viral gene expression by several mechanisms including direct and indirect binding to target viral promoters, synergy with EBV ZEBRA protein, and stimulation of cellular signaling pathways. We previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.022
更新日期:2009-04-10 00:00:00
abstract::The retroviral cyclin protein (rv-cyclin) of walleye dermal sarcoma virus contains two known functional domains, a cyclin box motif and a carboxy terminal transcription activation domain (AD). The AD contacts TATA-binding protein-associated factor 9 (TAF9), and this action is necessary for both positive and negative r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.026
更新日期:2009-03-30 00:00:00
abstract::Human herpesvirus 6 (HHV-6) is a ubiquitous betaherpesvirus that predominantly infects and replicates in CD4(+) T lymphocytes. However, the mechanism of HHV-6 transmission to T cells from the peripheral mucosa is unknown. Here we found that dendritic cells (DCs) can transmit HHV-6 to T cells, resulting in productive i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.049
更新日期:2009-03-15 00:00:00
abstract::The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.027
更新日期:2009-03-15 00:00:00
abstract::Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.032
更新日期:2009-03-15 00:00:00
abstract::Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.029
更新日期:2009-03-15 00:00:00
abstract::Friend murine leukemia virus (Fr-MLV) produces unspliced mRNAs, as well as singly-spliced mRNAs by which the Env protein is generated. We investigated the role of the intron within the Fr-MLV gene in Env expression using vectors with serially truncated introns. The up-regulatory regions, the 361-878 and the 5135-5399 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.040
更新日期:2009-03-01 00:00:00
abstract::HIV-1 genomic RNA (gRNA) dimerization is important for viral infectivity and is regulated by proteolytic processing of the Gag precursor protein (Pr55gag) under the direction of the viral protease. The processing occurs in successive steps and, to date, the step associated with formation of a wild-type (WT) level of g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.028
更新日期:2009-03-01 00:00:00
abstract::It is unknown which HIV proteins to target by vaccination in order to generate the most effective CD8 T-cell immunity. We recently immunized SIV(mac251)-infected pigtail macaques with Gag peptides or a cocktail of peptides spanning all SIV proteins, including SIV Env. High-level SIV Env-specific CD8 T-cell responses w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.030
更新日期:2009-02-05 00:00:00
abstract::Cucumber Mosaic Virus (CMV) is a plant infecting tripartite positive-strand RNA virus. In addition to three genomic and two known subgenomic RNAs, CMV strains of subgroup II (e.g. Q-CMV), but not subgroup I (e.g. Fny-CMV), produce and package a redundant RNA5 encompassing the 3' 304-307 nucleotides of RNAs 2 and 3. Th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.10.023
更新日期:2009-02-05 00:00:00